Coronaviruses are single-stranded RNA viruses that belong to the Coronaviridae family. It is the emergence of the most current coronaviruses, SARS-CoV-2 (the virus responsible for the disease Covid-19), which has the research community’s attention. The high infection and mortality rates has made the development of testing kits and a vaccine paramount.
COVID-19 consists of four major proteins including a protruding glycoprotein (spike), membrane, envelope, and nucleocapsid. Given its multi-protein structure scientists are utilizing EIA immunoassays, ELISA immunoassays, reverse transcription PC assays (RT-PCR) and real-time quantitative PCR assays (qRT-PCR).
The Centers for Disease Control and Prevention (CDC) is providing test kits for public health laboratories (PHLs) in order to perform real-time RT-polymerase chain reaction (rRT-PCR) detection for SARS-CoV-2. [1] Full protocol for CDC 2019-Novel Coronavirus (2019-nCoV) is available for download and intended for the presumptive qualitative detection of nucleic acid from the 2019-nCoV. [2]
Coronavirus assays and various staple lab equipment products are utilized in coronavirus testing which includes, but is not limited to:
References:
[1] Information for Laboratories: 2019-NCoV | CDC. 9 Feb. 2020, https://www.cdc.gov/coronavirus/2019-ncov/lab/index.html.
[2] CDC 2019-Novel Coronavirus (2019-nCoV) Real-Time RT-PCR Diagnostic Panel. https://www.fda.gov/media/134922/downloa